Identification of novel subgenomic RNAs and noncanonical transcription initiation signals of severe acute respiratory syndrome coronavirus
Identifieur interne : 004E13 ( Main/Exploration ); précédent : 004E12; suivant : 004E14Identification of novel subgenomic RNAs and noncanonical transcription initiation signals of severe acute respiratory syndrome coronavirus
Auteurs : Snawar Hussain [République populaire de Chine] ; JI'AN PAN [République populaire de Chine] ; YU CHEN [République populaire de Chine] ; YALIN YANG [République populaire de Chine] ; JING XU [République populaire de Chine] ; YU PENG [République populaire de Chine] ; YING WU [République populaire de Chine] ; ZHAOYANG LI [République populaire de Chine] ; YING ZHU [République populaire de Chine] ; Po Tien [République populaire de Chine] ; DEYIN GUO [République populaire de Chine]Source :
- Journal of virology [ 0022-538X ] ; 2005.
Descripteurs français
- KwdFr :
- ARN messager (génétique), ARN viral (biosynthèse), ARN viral (génétique), Animaux, Cadres ouverts de lecture, Cellules Vero, Cricetinae, Données de séquences moléculaires, Génome viral, Matrices (génétique), Site d'initiation de la transcription, Séquence nucléotidique, Transcription génétique, Virus du SRAS (génétique).
- MESH :
- biosynthèse : ARN viral.
- génétique : ARN messager, ARN viral, Virus du SRAS.
- Pascal (Inist)
- Animaux, Cadres ouverts de lecture, Cellules Vero, Coronavirus, Cricetinae, Données de séquences moléculaires, Génome viral, Identification, Initiation transcription, Matrices (génétique), Microbiologie, Site d'initiation de la transcription, Séquence nucléotidique, Transcription génétique, Virologie, Syndrome respiratoire aigu sévère.
English descriptors
- KwdEn :
- Animals, Base Sequence, Chlorocebus aethiops, Coronavirus, Cricetinae, Genome, Viral, Identification, Microbiology, Molecular Sequence Data, Open Reading Frames, RNA, Messenger (genetics), RNA, Viral (biosynthesis), RNA, Viral (genetics), SARS Virus (genetics), Severe acute respiratory syndrome, Templates, Genetic, Transcription Initiation Site, Transcription initiation, Transcription, Genetic, Vero Cells, Virology.
- MESH :
- chemical , biosynthesis : RNA, Viral.
- chemical , genetics : RNA, Messenger, RNA, Viral.
- genetics : SARS Virus.
- Animals, Base Sequence, Chlorocebus aethiops, Cricetinae, Genome, Viral, Molecular Sequence Data, Open Reading Frames, Templates, Genetic, Transcription Initiation Site, Transcription, Genetic, Vero Cells.
Abstract
The expression of the genomic information of severe acute respiratory syndrome coronavirus (SARS CoV) involves synthesis of a nested set of subgenomic RNAs (sgRNAs) by discontinuous transcription. In SARS CoV-infected cells, 10 sgRNAs, including 2 novel ones, were identified, which were predicted to be functional in the expression of 12 open reading frames located in the 3' one-third of the genome. Surprisingly, one new sgRNA could lead to production of a truncated spike protein. Sequence analysis of the leader-body fusion sites of each sgRNA showed that the junction sequences and the corresponding transcription-regulatory sequence (TRS) are unique for each species of sgRNA and are consistent after virus passages. For the two novel sgRNAs, each used a variant of the TRS that has one nucleotide mismatch in the conserved hexanucleotide core (ACGAAC) in the TRS. Coexistence of both plus and minus strands of SARS CoV sgRNAs and evidence for derivation of the sgRNA core sequence from the body core sequence favor the model of discontinuous transcription during minus-strand synthesis. Moreover, one rare species of sgRNA has the junction sequence AAA, indicating that its transcription could result from a noncanonical transcription signal. Taken together, these results provide more insight into the molecular mechanisms of genome expression and subgenomic transcription of SARS CoV.
Url:
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PascalFrancis, to step Corpus: 000697
- to stream PascalFrancis, to step Curation: 000293
- to stream PascalFrancis, to step Checkpoint: 000633
- to stream Main, to step Merge: 005153
- to stream Pmc, to step Corpus: 000692
- to stream Pmc, to step Curation: 000692
- to stream Pmc, to step Checkpoint: 001326
- to stream PubMed, to step Corpus: 002795
- to stream PubMed, to step Curation: 002795
- to stream PubMed, to step Checkpoint: 002674
- to stream Ncbi, to step Merge: 000E90
- to stream Ncbi, to step Curation: 000E90
- to stream Ncbi, to step Checkpoint: 000E90
- to stream Main, to step Merge: 004A98
- to stream Main, to step Curation: 004E13
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Identification of novel subgenomic RNAs and noncanonical transcription initiation signals of severe acute respiratory syndrome coronavirus</title><author><name sortKey="Hussain, Snawar" sort="Hussain, Snawar" uniqKey="Hussain S" first="Snawar" last="Hussain">Snawar Hussain</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Modern Virology Research Centre and National Key Laboratory of urology, College of Life Sciences, Wuhan University</s1><s2>Wuhan</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName><orgName type="university">Université de Wuhan</orgName></affiliation></author><author><name sortKey="Ji An Pan" sort="Ji An Pan" uniqKey="Ji An Pan" last="Ji'An Pan">JI'AN PAN</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Modern Virology Research Centre and National Key Laboratory of urology, College of Life Sciences, Wuhan University</s1><s2>Wuhan</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName><orgName type="university">Université de Wuhan</orgName></affiliation></author><author><name sortKey="Yu Chen" sort="Yu Chen" uniqKey="Yu Chen" last="Yu Chen">YU CHEN</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Modern Virology Research Centre and National Key Laboratory of urology, College of Life Sciences, Wuhan University</s1><s2>Wuhan</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName><orgName type="university">Université de Wuhan</orgName></affiliation></author><author><name sortKey="Yalin Yang" sort="Yalin Yang" uniqKey="Yalin Yang" last="Yalin Yang">YALIN YANG</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Modern Virology Research Centre and National Key Laboratory of urology, College of Life Sciences, Wuhan University</s1><s2>Wuhan</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName><orgName type="university">Université de Wuhan</orgName></affiliation></author><author><name sortKey="Jing Xu" sort="Jing Xu" uniqKey="Jing Xu" last="Jing Xu">JING XU</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Modern Virology Research Centre and National Key Laboratory of urology, College of Life Sciences, Wuhan University</s1><s2>Wuhan</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName><orgName type="university">Université de Wuhan</orgName></affiliation></author><author><name sortKey="Yu Peng" sort="Yu Peng" uniqKey="Yu Peng" last="Yu Peng">YU PENG</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Modern Virology Research Centre and National Key Laboratory of urology, College of Life Sciences, Wuhan University</s1><s2>Wuhan</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName><orgName type="university">Université de Wuhan</orgName></affiliation></author><author><name sortKey="Ying Wu" sort="Ying Wu" uniqKey="Ying Wu" last="Ying Wu">YING WU</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Modern Virology Research Centre and National Key Laboratory of urology, College of Life Sciences, Wuhan University</s1><s2>Wuhan</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName><orgName type="university">Université de Wuhan</orgName></affiliation></author><author><name sortKey="Zhaoyang Li" sort="Zhaoyang Li" uniqKey="Zhaoyang Li" last="Zhaoyang Li">ZHAOYANG LI</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Modern Virology Research Centre and National Key Laboratory of urology, College of Life Sciences, Wuhan University</s1><s2>Wuhan</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName><orgName type="university">Université de Wuhan</orgName></affiliation></author><author><name sortKey="Ying Zhu" sort="Ying Zhu" uniqKey="Ying Zhu" last="Ying Zhu">YING ZHU</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Modern Virology Research Centre and National Key Laboratory of urology, College of Life Sciences, Wuhan University</s1><s2>Wuhan</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName><orgName type="university">Université de Wuhan</orgName></affiliation></author><author><name sortKey="Tien, Po" sort="Tien, Po" uniqKey="Tien P" first="Po" last="Tien">Po Tien</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Modern Virology Research Centre and National Key Laboratory of urology, College of Life Sciences, Wuhan University</s1><s2>Wuhan</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName><orgName type="university">Université de Wuhan</orgName></affiliation></author><author><name sortKey="Deyin Guo" sort="Deyin Guo" uniqKey="Deyin Guo" last="Deyin Guo">DEYIN GUO</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Modern Virology Research Centre and National Key Laboratory of urology, College of Life Sciences, Wuhan University</s1><s2>Wuhan</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName><orgName type="university">Université de Wuhan</orgName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">05-0209520</idno><date when="2005">2005</date><idno type="stanalyst">PASCAL 05-0209520 INIST</idno><idno type="RBID">Pascal:05-0209520</idno><idno type="wicri:Area/PascalFrancis/Corpus">000697</idno><idno type="wicri:Area/PascalFrancis/Curation">000293</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000633</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000633</idno><idno type="wicri:doubleKey">0022-538X:2005:Hussain S:identification:of:novel</idno><idno type="wicri:Area/Main/Merge">005153</idno><idno type="wicri:source">PMC</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1082772</idno><idno type="RBID">PMC:1082772</idno><idno type="wicri:Area/Pmc/Corpus">000692</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000692</idno><idno type="wicri:Area/Pmc/Curation">000692</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000692</idno><idno type="wicri:Area/Pmc/Checkpoint">001326</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">001326</idno><idno type="wicri:source">PubMed</idno><idno type="RBID">pubmed:15827143</idno><idno type="wicri:Area/PubMed/Corpus">002795</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002795</idno><idno type="wicri:Area/PubMed/Curation">002795</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002795</idno><idno type="wicri:Area/PubMed/Checkpoint">002674</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002674</idno><idno type="wicri:Area/Ncbi/Merge">000E90</idno><idno type="wicri:Area/Ncbi/Curation">000E90</idno><idno type="wicri:Area/Ncbi/Checkpoint">000E90</idno><idno type="wicri:doubleKey">0022-538X:2005:Hussain S:identification:of:novel</idno><idno type="wicri:Area/Main/Merge">004A98</idno><idno type="wicri:Area/Main/Curation">004E13</idno><idno type="wicri:Area/Main/Exploration">004E13</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Identification of novel subgenomic RNAs and noncanonical transcription initiation signals of severe acute respiratory syndrome coronavirus</title><author><name sortKey="Hussain, Snawar" sort="Hussain, Snawar" uniqKey="Hussain S" first="Snawar" last="Hussain">Snawar Hussain</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Modern Virology Research Centre and National Key Laboratory of urology, College of Life Sciences, Wuhan University</s1><s2>Wuhan</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName><orgName type="university">Université de Wuhan</orgName></affiliation></author><author><name sortKey="Ji An Pan" sort="Ji An Pan" uniqKey="Ji An Pan" last="Ji'An Pan">JI'AN PAN</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Modern Virology Research Centre and National Key Laboratory of urology, College of Life Sciences, Wuhan University</s1><s2>Wuhan</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName><orgName type="university">Université de Wuhan</orgName></affiliation></author><author><name sortKey="Yu Chen" sort="Yu Chen" uniqKey="Yu Chen" last="Yu Chen">YU CHEN</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Modern Virology Research Centre and National Key Laboratory of urology, College of Life Sciences, Wuhan University</s1><s2>Wuhan</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName><orgName type="university">Université de Wuhan</orgName></affiliation></author><author><name sortKey="Yalin Yang" sort="Yalin Yang" uniqKey="Yalin Yang" last="Yalin Yang">YALIN YANG</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Modern Virology Research Centre and National Key Laboratory of urology, College of Life Sciences, Wuhan University</s1><s2>Wuhan</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName><orgName type="university">Université de Wuhan</orgName></affiliation></author><author><name sortKey="Jing Xu" sort="Jing Xu" uniqKey="Jing Xu" last="Jing Xu">JING XU</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Modern Virology Research Centre and National Key Laboratory of urology, College of Life Sciences, Wuhan University</s1><s2>Wuhan</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName><orgName type="university">Université de Wuhan</orgName></affiliation></author><author><name sortKey="Yu Peng" sort="Yu Peng" uniqKey="Yu Peng" last="Yu Peng">YU PENG</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Modern Virology Research Centre and National Key Laboratory of urology, College of Life Sciences, Wuhan University</s1><s2>Wuhan</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName><orgName type="university">Université de Wuhan</orgName></affiliation></author><author><name sortKey="Ying Wu" sort="Ying Wu" uniqKey="Ying Wu" last="Ying Wu">YING WU</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Modern Virology Research Centre and National Key Laboratory of urology, College of Life Sciences, Wuhan University</s1><s2>Wuhan</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName><orgName type="university">Université de Wuhan</orgName></affiliation></author><author><name sortKey="Zhaoyang Li" sort="Zhaoyang Li" uniqKey="Zhaoyang Li" last="Zhaoyang Li">ZHAOYANG LI</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Modern Virology Research Centre and National Key Laboratory of urology, College of Life Sciences, Wuhan University</s1><s2>Wuhan</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName><orgName type="university">Université de Wuhan</orgName></affiliation></author><author><name sortKey="Ying Zhu" sort="Ying Zhu" uniqKey="Ying Zhu" last="Ying Zhu">YING ZHU</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Modern Virology Research Centre and National Key Laboratory of urology, College of Life Sciences, Wuhan University</s1><s2>Wuhan</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName><orgName type="university">Université de Wuhan</orgName></affiliation></author><author><name sortKey="Tien, Po" sort="Tien, Po" uniqKey="Tien P" first="Po" last="Tien">Po Tien</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Modern Virology Research Centre and National Key Laboratory of urology, College of Life Sciences, Wuhan University</s1><s2>Wuhan</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName><orgName type="university">Université de Wuhan</orgName></affiliation></author><author><name sortKey="Deyin Guo" sort="Deyin Guo" uniqKey="Deyin Guo" last="Deyin Guo">DEYIN GUO</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Modern Virology Research Centre and National Key Laboratory of urology, College of Life Sciences, Wuhan University</s1><s2>Wuhan</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>République populaire de Chine</country><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName><orgName type="university">Université de Wuhan</orgName></affiliation></author></analytic><series><title level="j" type="main">Journal of virology</title><title level="j" type="abbreviated">J. virol.</title><idno type="ISSN">0022-538X</idno><imprint><date when="2005">2005</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of virology</title><title level="j" type="abbreviated">J. virol.</title><idno type="ISSN">0022-538X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Base Sequence</term><term>Chlorocebus aethiops</term><term>Coronavirus</term><term>Cricetinae</term><term>Genome, Viral</term><term>Identification</term><term>Microbiology</term><term>Molecular Sequence Data</term><term>Open Reading Frames</term><term>RNA, Messenger (genetics)</term><term>RNA, Viral (biosynthesis)</term><term>RNA, Viral (genetics)</term><term>SARS Virus (genetics)</term><term>Severe acute respiratory syndrome</term><term>Templates, Genetic</term><term>Transcription Initiation Site</term><term>Transcription initiation</term><term>Transcription, Genetic</term><term>Vero Cells</term><term>Virology</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN messager (génétique)</term><term>ARN viral (biosynthèse)</term><term>ARN viral (génétique)</term><term>Animaux</term><term>Cadres ouverts de lecture</term><term>Cellules Vero</term><term>Cricetinae</term><term>Données de séquences moléculaires</term><term>Génome viral</term><term>Matrices (génétique)</term><term>Site d'initiation de la transcription</term><term>Séquence nucléotidique</term><term>Transcription génétique</term><term>Virus du SRAS (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>RNA, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>RNA, Messenger</term><term>RNA, Viral</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>ARN viral</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN messager</term><term>ARN viral</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Base Sequence</term><term>Chlorocebus aethiops</term><term>Cricetinae</term><term>Genome, Viral</term><term>Molecular Sequence Data</term><term>Open Reading Frames</term><term>Templates, Genetic</term><term>Transcription Initiation Site</term><term>Transcription, Genetic</term><term>Vero Cells</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Animaux</term><term>Cadres ouverts de lecture</term><term>Cellules Vero</term><term>Coronavirus</term><term>Cricetinae</term><term>Données de séquences moléculaires</term><term>Génome viral</term><term>Identification</term><term>Initiation transcription</term><term>Matrices (génétique)</term><term>Microbiologie</term><term>Site d'initiation de la transcription</term><term>Séquence nucléotidique</term><term>Transcription génétique</term><term>Virologie</term><term>Syndrome respiratoire aigu sévère</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The expression of the genomic information of severe acute respiratory syndrome coronavirus (SARS CoV) involves synthesis of a nested set of subgenomic RNAs (sgRNAs) by discontinuous transcription. In SARS CoV-infected cells, 10 sgRNAs, including 2 novel ones, were identified, which were predicted to be functional in the expression of 12 open reading frames located in the 3' one-third of the genome. Surprisingly, one new sgRNA could lead to production of a truncated spike protein. Sequence analysis of the leader-body fusion sites of each sgRNA showed that the junction sequences and the corresponding transcription-regulatory sequence (TRS) are unique for each species of sgRNA and are consistent after virus passages. For the two novel sgRNAs, each used a variant of the TRS that has one nucleotide mismatch in the conserved hexanucleotide core (ACGAAC) in the TRS. Coexistence of both plus and minus strands of SARS CoV sgRNAs and evidence for derivation of the sgRNA core sequence from the body core sequence favor the model of discontinuous transcription during minus-strand synthesis. Moreover, one rare species of sgRNA has the junction sequence AAA, indicating that its transcription could result from a noncanonical transcription signal. Taken together, these results provide more insight into the molecular mechanisms of genome expression and subgenomic transcription of SARS CoV.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Hubei</li></region><settlement><li>Wuhan</li></settlement><orgName><li>Université de Wuhan</li></orgName></list><tree><country name="République populaire de Chine"><region name="Hubei"><name sortKey="Hussain, Snawar" sort="Hussain, Snawar" uniqKey="Hussain S" first="Snawar" last="Hussain">Snawar Hussain</name></region><name sortKey="Deyin Guo" sort="Deyin Guo" uniqKey="Deyin Guo" last="Deyin Guo">DEYIN GUO</name><name sortKey="Ji An Pan" sort="Ji An Pan" uniqKey="Ji An Pan" last="Ji'An Pan">JI'AN PAN</name><name sortKey="Jing Xu" sort="Jing Xu" uniqKey="Jing Xu" last="Jing Xu">JING XU</name><name sortKey="Tien, Po" sort="Tien, Po" uniqKey="Tien P" first="Po" last="Tien">Po Tien</name><name sortKey="Yalin Yang" sort="Yalin Yang" uniqKey="Yalin Yang" last="Yalin Yang">YALIN YANG</name><name sortKey="Ying Wu" sort="Ying Wu" uniqKey="Ying Wu" last="Ying Wu">YING WU</name><name sortKey="Ying Zhu" sort="Ying Zhu" uniqKey="Ying Zhu" last="Ying Zhu">YING ZHU</name><name sortKey="Yu Chen" sort="Yu Chen" uniqKey="Yu Chen" last="Yu Chen">YU CHEN</name><name sortKey="Yu Peng" sort="Yu Peng" uniqKey="Yu Peng" last="Yu Peng">YU PENG</name><name sortKey="Zhaoyang Li" sort="Zhaoyang Li" uniqKey="Zhaoyang Li" last="Zhaoyang Li">ZHAOYANG LI</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004E13 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 004E13 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= Pascal:05-0209520
|texte= Identification of novel subgenomic RNAs and noncanonical transcription initiation signals of severe acute respiratory syndrome coronavirus
}}
| This area was generated with Dilib version V0.6.33. |  |